| Place | Performance | Athlete | Team |
| 1 | 24.44 | VAN DER MERWE - DENE | AFRIKAANS HOR MEISIES PRETORIA |
| 2 | 24.64 | VAN DER WESTHUIZEN - EMMA - LEE | DIE HORSKOOL MENLOPARK |
| 3 | 24.74 | MAAKE - MOKGADI | CURRO HAZELDEAN |
| 4 | 25.98 | VAN DER WESTHUIZEN - LANE | HORSKOOL WATERKLOOF |
| 5 | 26.14 | ROOS - LUZELLE | HORSKOOL ZWARTKOP |
| 6 | 26.30 | STEENKAMP - DANIKA | HORSKOOL GARSFONTEIN |
| 7 | 26.44 | JACOBS - ELNIKA | HORSKOOL MONTANA |
| 8 | 26.95 | VENTER - CARLI | MIDSTREAM COLLEGE |