| Place | Performance | Athlete | Team |
| 1 | 42.83 | SWANEPOEL - LI-MAY | AFRIKAANS HOR MEISIES PRETORIA |
| 2 | 43.13 | MOOLMAN - MILANE | DIE HORSKOOL MENLOPARK |
| 3 | 44.39 | VAN ROOYEN - TYLER | HORSKOOL ZWARTKOP |
| 4 | 46.60 | LOMBARD - ANGELIQUE | MIDSTREAM COLLEGE |
| 5 | 46.76 | GERBER - ABIGAIL | HORSKOOL MONTANA |
| 6 | 47.65 | DU PLOOY - ALDI | HORSKOOL WATERKLOOF |
| 7 | 50.28 | RAMOKGADI - REMORATILE | CURRO HAZELDEAN |
| 8 | 50.92 | VAN WYK - RIHETTE | HORSKOOL GARSFONTEIN |