| Place | Performance | Athlete | Team |
| 1 | 0:49.16 | -: RELAY TEAM - TUKSSPORT HS | TUKS SPORT HIGH SCHOOL |
| 2 | 0:51.33 | q: RELAY TEAM - MIDSTREAM | MIDSTREAM COLLEGE |
| 3 | 0:53.00 | -: RELAY TEAM - WATERKLOOF | WATERKLOOF HORSKOOL |
| 4 | 0:55.58 | -: RELAY TEAM - PTA GIRLS HIGH | PRETORIA HIGH SCHOOL FOR GIRLS |
| 5 | 0:56.01 | -: RELAY TEAM - JEUGLAND | JEUGLAND |
| 6 | 1:01.27 | -: RELAY TEAM - WOODHILL COLLEGE | WOODHILL COLLEGE |