| Place | Performance | Athlete | Team |
| 1 | 09.34 | q: DU TOIT - KLARA | MENLOPARK LAERSKOOL |
| 2 | 09.53 | q: POLLOCK - TIFFANY | PRETORIA OOS LAERSKOOL |
| 3 | 09.73 | q: JACOBS - HANNA | ANTON VAN WOUW LAERSKOOL |
| 4 | 09.79 | -: LOUWIES - CHENE | CONSTANTIAPARK LAERSKOOL |
| 5 | 09.93 | -: OPPERMAN - LON | SKUILKRANS LAERSKOOL |
| 6 | 09.97 | -: MALAN - LIVI | MONUMENTPARK LAERSKOOL |