| Place | Performance | Athlete | Team |
| 1 | 21.12 | q: LABUSCHAGNE - HAYLIE | GARSFONTEIN LAERSKOOL |
| 2 | 21.49 | q: JANSE VAN RENSBURG - NOELLE | ELARDUSPARK LAERSKOOL |
| 3 | 21.84 | q: PEPENENE - LEBO | LYNNWOOD LAERSKOOL |
| 4 | 22.43 | -: DE JAGER - KAILAH | PRETORIA OOS LAERSKOOL |
| 5 | 22.78 | -: BEYL - OLIVIA | MONUMENTPARK LAERSKOOL |
| 6 | 22.87 | -: MORE - HAILEY | CONSTANTIAPARK LAERSKOOL |