| Place | Performance | Athlete | Team |
| 1 | 23.78 | -: MOLEPO - PRECIOUS | UNIVERSITY OF JOHANNESBURG |
| 2 | 25.38 | -: BATISANI - KENNEKAE | BOTSWANA |
| 3 | 25.43 | q: MAKASI - LOLWETHU | UNIVERSITY OF JOHANNESBURG |
| 4 | 25.81 | q: KOTZE - DANE | UNIVERSITY OF JOHANNESBURG |
| 5 | 28.81 | -: MULUSO - NATASHA | ESWATINI |
| 6 | 28.95 | -: DLAMINI - NOTHANDO | ESWATINI |