| Place | Performance | Athlete | Team |
| 1 | 0:47.00 | q: NZWANZWA - ANELE | THSWANE UNIVERSITY OF TECHNOLOGY |
| 2 | 0:47.41 | q: MESHACK - CLIFFTON | BOTSWANA |
| 3 | 0:47.70 | q: KOOLOPILE - ONALENNA | BOTSWANA |
| 4 | 0:49.35 | q: NDONGENI - LIKHO | HEIDENDAL |
| 5 | 0:49.61 | q: QUITE - TAWANA M G E | UBAC CLUB |
| 6 | 0:49.85 | q: TLHAPI - TSHEPISO | UNIVERSITY OF JOHANNESBURG |
| 7 | 0:50.90 | q: LEKWALO - MPHO JASON | BOTSWANA |
| 8 | 0:51.75 | -: MULUMBA - JACY | MASAI AC |