| Place | Performance | Athlete | Team |
| 1 | 0:46.27 | -: MUWISHI - GERREN | ZIMBABWE |
| 2 | 0:46.89 | -: MASILO - BOITUMELO | BOTSWANA |
| 3 | 0:47.76 | -: REETSANG - GIVEN | BOTSWANA |
| 4 | 0:48.67 | q: REHLAMFU - MUSA | TUKS ATHLETICS CLUB |
| 5 | 0:49.19 | -: KALASIYA - TAKUDZWA | ZIMBABWE |
| 6 | 0:49.54 | -: NGAVI - DENZEL | ZIMBABWE |
| 7 | 0:50.12 | q: TLHOMELANG - KUTLO | JWANENG ATHLETIC CLUB |
| 8 | 0:52.32 | q: MAVUNDA - EVANS | UNIVERSITY OF JOHANNESBURG |