| Place | Performance | Athlete | Team |
| 1 | 0:46.00 | -: ZUZE - LEEFORD | ZIMBABWE |
| 2 | 0:46.35 | -: MOHOLOBELA - KABELO | FITNESS FROM AFRICA |
| 3 | 0:49.07 | q: DU PLESSIS - BEN | TUKS ATHLETICS CLUB |
| 4 | 0:49.15 | q: MARUMA - LEEMA | PHOBIANS |
| 5 | 0:49.95 | -: RAMOTHWALA - KGOTHATSO | MEGA AC |
| 6 | 0:50.02 | -: KEKGATHETSE - TEFO | BOTSWANA |