| Place | Performance | Athlete | Team |
| 1 | 0:53.97 | q: SENAUOANE - MAMAKOLI | LESOTHO |
| 2 | 0:55.73 | q: HATTINGH - CARLA | NORTH WEST UNIVERSITY |
| 3 | 0:55.99 | q: RANTE - MOTLATSI | BOTSWANA |
| 4 | 0:57.24 | q: SENYAMA - GAOLAPE | BOTSWANA |
| 5 | 0:59.50 | q: SAKAROMBE - MONIQUE | UNIVERSITY OF JOHANNESBURG |
| 6 | 1:03.12 | q: MONYOBO - PINKI PATRICIA | LESOTHO |
| 7 | 1:08.41 | q: MANASWE - MOKHUTSO | MASAI AC |