| Place | Performance | Athlete | Team |
| 1 | 0:54.71 | q: BASELE - THOMPHANG | BOTSWANA |
| 2 | 0:55.62 | -: MONTHE - NALEDI | BOTSWANA |
| 3 | 0:56.09 | q: STRONG - DAVIDA | I HAVE TALENT (IHT) |
| 4 | 0:57.02 | q: SELEKA - BOITUMELO | ROYAL BAFOKENG ATHLETIC TEAM |
| 5 | 0:58.47 | -: JACK - RITA | BOTSWANA |
| 6 | 0:59.59 | q: KOTZE - DANE | UNIVERSITY OF JOHANNESBURG |
| 7 | 1:00.37 | q: MATIWE - WEDU | BOTSWANA |
| 8 | 1:04.26 | q: CHAKALISA - MAGDELINE | BOTSWANA |