| Place | Performance | Athlete | Team |
| 1 | 0:55.11 | q: BATISANI - KENNEKAE | BOTSWANA |
| 2 | 0:55.23 | q: MOROKO - GALEFELE | BOTSWANA |
| 3 | 0:55.46 | q: STRIJDOM - ANGELIQUE | THSWANE UNIVERSITY OF TECHNOLOGY |
| 4 | 0:56.08 | q: VILJOEN - MARIETJIE | TUKS ATHLETICS CLUB |
| 5 | 0:56.36 | q: EZE - DIANA | UNIVERSITY OF JOHANNESBURG |
| 6 | 1:05.21 | -: VILJOEN - CORLEY | NAMIBIA |
| 7 | 1:07.54 | -: NIKISI - TRISH VIMBAI | ZIMBABWE |