| Place | Performance | Athlete | Team |
| 1 | 10.27 | q: MLENGA - RETSHIDISITSWE | UNIVERSITY OF JOHANNESBURG |
| 2 | 10.65 | q: MADI - MICHAEL | UNIVERSITY OF JOHANNESBURG |
| 3 | 10.72 | q: SALIWE - REITUMETSE | GOING4GOLD |
| 4 | 10.92 | -: MAZIYA - MLANDVO | ESWATINI |
| 5 | 11.02 | q: NKOSI - SIYA | TUKS ATHLETICS CLUB |
| 6 | 11.16 | q: TLADI - SFISO | MEGA AC |