| Place | Performance | Athlete | Team |
| 1 | 11.86 | q: AYANLEKE - STELLA | UNIVERSITY OF JOHANNESBURG |
| 2 | 12.05 | q: WANSBURY - CHARNE | TUKS ATHLETICS CLUB |
| 3 | 12.07 | q: FAGBEMI - AMEENAT | UNIVERSITY OF JOHANNESBURG |
| 4 | 12.27 | q: STOLZ - JENNA | UNIVERSITY OF JOHANNESBURG |
| 5 | 12.60 | q: ODINWAMKPA - ADAORA DIOR | UNIVERSITY OF JOHANNESBURG |
| 6 | 12.85 | q: TAYLOR - ALYSSA | KOVSIE ATHLETICS |
| 7 | 12.90 | q: NOMPOZOLO - BATHABILE | TUKS ATHLETICS CLUB |