| Place | Performance | Athlete | Team |
| 1 | 25.52 | Q: ORJI - ISOBEL | ATHLETICS FREE STATE |
| 2 | 25.72 | Q: JORDAAN - JUANE | ATHLETICS GAUTENG NORTH |
| 3 | 26.10 | Q: KORFF - ROEMAY | ATHLETICS GAUTENG NORTH |
| 4 | 26.71 | Q: ESTERHUYSEN - CORNELIA | BOLAND ATHLETICS |
| 5 | 26.76 | q: MAJETJA - NTOMBI | CENTRAL GAUTENG ATHLETICS |
| 6 | 28.34 | -: STRYDOM - LIENKA | ATHLETICS VAAL TRIANGLE |
| 7 | 30.04 | -: MVITSHANA - SANDA | ATHLETICS TRANSKEI |