| Place | Performance | Athlete | Team |
| 1 | 0:48.25 | Q: VAN DER LINDE - JANU | BOLAND ATHLETICS |
| 2 | 0:48.61 | Q: DU PLESSIS - LIAM | CENTRAL GAUTENG ATHLETICS |
| 3 | 0:49.30 | Q: VAN DER WALT - MARCUS | ATHLETICS GAUTENG NORTH |
| 4 | 0:49.31 | Q: STAFA - LUKHANYO | ATHLETICS FREE STATE |
| 5 | 0:49.63 | -: NDLOVU - MTHOKOZISI | ATHLETICS MPUMALANGA |
| 6 | 0:49.86 | -: SIBEKO - NHLAKANIPHO | CENTRAL GAUTENG ATHLETICS |
| 7 | 0:49.93 | -: KOENA - OARABILE | ATHLETICS CENTRAL NORTHWEST |