| Place | Performance | Athlete | Team |
| 1 | 0:47.91 | Q: MAHLANGU - SIHLE | ATHLETICS CENTRAL NORTHWEST |
| 2 | 0:48.72 | Q: CHAMBERS - JOHANN | CENTRAL GAUTENG ATHLETICS |
| 3 | 0:48.90 | Q: MARUMA - LEEMA | ATHLETICS GAUTENG NORTH |
| 4 | 0:49.09 | Q: MAAMOGAWA - THUTO | CENTRAL GAUTENG ATHLETICS |
| 5 | 0:50.20 | -: XABA - ANDILE | KWAZULU-NATAL ATHLETICS |
| 6 | 0:51.10 | -: MASHABELA - LEHLOHONOLO | ATHLETICS GAUTENG NORTH |
| 7 | -: DU PLESSIS - JUAN | ATHLETICS CENTRAL NORTHWEST |