| Place | Performance | Athlete | Team |
| 1 | 0:55.24 | Q: SCHEEPERS - COLENE | ATHLETICS GAUTENG NORTH |
| 2 | 0:55.63 | Q: WILSON - MIA | BOLAND ATHLETICS |
| 3 | 0:56.12 | q: ESTERHUYSE - VENE | CENTRAL GAUTENG ATHLETICS |
| 4 | 0:58.31 | -: SHIPALANA - PENELOPE | CENTRAL GAUTENG ATHLETICS |
| 5 | 0:58.36 | -: PHALANE - ROSINA | LIMPOPO ATHLETICS |
| 6 | 0:59.22 | -: REDDY - KEIRA | KWAZULU-NATAL ATHLETICS |