| Place | Performance | Athlete | Team |
| 1 | 12.43 | Q: MAAKE - MOKGADI | ATHLETICS GAUTENG NORTH |
| 2 | 12.53 | Q: MAGWAZA - BUSISIWE | ATHLETICS GAUTENG NORTH |
| 3 | 12.54 | Q: MAKHANYA - FUNDISWA | ATHLETICS GAUTENG NORTH |
| 4 | 12.55 | Q: NEL - AMBER | BOLAND ATHLETICS |
| 5 | 12.62 | -: ESTERHUYSEN - CORNELIA | BOLAND ATHLETICS |
| 6 | 13.05 | -: NDJEBO - SIPHESIHLE | CENTRAL GAUTENG ATHLETICS |
| 7 | 13.18 | -: DAVIDS - ELISHA | WESTERN PROVINCE ATHLETICS |