| Place | Performance | Athlete | Team |
| 1 | 0:47.31 | Q: MAHLANGU - SIHLE | ATHLETICS CENTRAL NORTHWEST |
| 2 | 0:47.32 | Q: UDEME - OKON | CENTRAL GAUTENG ATHLETICS |
| 3 | 0:48.01 | Q: SEMENYA - JOEL | ATHLETICS GAUTENG NORTH |
| 4 | 0:48.57 | -: HLATSHWAYO - MILDO | KWAZULU-NATAL ATHLETICS |
| 5 | 0:48.81 | -: VAN HEERDEN - LLEWELLYN | ATHLETICS GAUTENG NORTH |
| 6 | 0:48.93 | -: LOTIKA - KADMIEL | LIMPOPO ATHLETICS |