| Place | Performance | Athlete | Team |
| 1 | 0:54.38 | Q: MOLEPO - PRECIOUS | CENTRAL GAUTENG ATHLETICS |
| 2 | 0:54.55 | Q: STRYDOM - ANGELIQUE | ATHLETICS GAUTENG NORTH |
| 3 | 0:54.56 | -: VAN STADEN - JADA | ATHLETICS GAUTENG NORTH |
| 4 | 0:56.03 | -: VILJOEN - MARIETJIE | ATHLETICS GAUTENG NORTH |
| 5 | 0:58.51 | -: VAN HEERDEN - JESSICA | ATHLETICS CENTRAL NORTHWEST |
| 6 | 1:00.45 | -: THOMSEN - MICHAELA | ATHLETICS CENTRAL NORTHWEST |