| Place | Performance | Athlete | Team |
| 1 | 03:53.86 | Q: RELAY TEAM - UJ B TEAM | UNIVERSITY OF JOHANNESBURG B |
| 2 | 04:00.82 | Q: RELAY TEAM - TUKS B1 TEAM | UNIVERSITY OF PRETORIA B TEAM |
| 3 | 04:05.56 | Q: RELAY TEAM - TUKS A 2 TEAM | UNIVERSITY OF PRETORIA A TEAM |
| 4 | 04:25.18 | -: RELAY TEAM - VAAL UNIVERSITY | VAAL UNIVERSITY OF TECHNOLOGY |