| Place | Performance | Athlete | Team |
| 1 | 23.98 | Q: VERMEULEN - CHANE | UNIVERSITY OF JOHANNESBURG A |
| 2 | 24.07 | Q: SMIDT - CHARDONE | NORTH WEST UNIVERSITY POTCH A |
| 3 | 24.13 | q: COMPAAN - MILA | UNIVERSITY OF PRETORIA B TEAM |
| 4 | 24.30 | -: ANGELIQUE - STRYDOM | TSHWANE UNIV OF TECHNOLOGY |
| 5 | 24.88 | -: FAGBEMI - AMEENAT | UNIVERSITY OF JOHANNESBURG A |
| 6 | 25.07 | -: DE BEER - MIA | UNIVERSITY OF JOHANNESBURG B |
| 7 | 25.19 | -: BAYMAN - LAURE | UNIVERSITY OF WESTERN CAPE |