| Place | Performance | Athlete | Team |
| 1 | 23.94 | Q: MARAIS - GABRIELLA | UNIVERSITY OF FREE STATE |
| 2 | 24.10 | Q: GEORGE - ANNESTAYSHA | UNIVERSITY OF PRETORIA A TEAM |
| 3 | 24.41 | -: SCHEEPERS - COLENE | UNIVERSITY OF PRETORIA A TEAM |
| 4 | 24.45 | -: VILANKULU - ALUWANI | UNIVERSITY OF JOHANNESBURG A |
| 5 | 24.69 | -: GERMISHUYEN - EMMARENTIA | UNIVERSITY OF JOHANNESBURG A |
| 6 | 25.18 | -: COLLINS - YAVE | UNIVERSITY OF PRETORIA B TEAM |
| 7 | 25.44 | -: KOEKEMOER - DAYLE | VARSITY COLLEGE |