| Place | Performance | Athlete | Team |
| 1 | 24.28 | Q: HAITEMBU - NDAWANA | UNIVERSITY OF JOHANNESBURG A |
| 2 | 24.32 | Q: BOTJE - KAILIE | NORTH WEST UNIVERSITY POTCH A |
| 3 | 24.62 | Q: VILANKULU - ALUWANI | UNIVERSITY OF JOHANNESBURG A |
| 4 | 25.15 | -: ALIYAH - MBOMBO | UNIVERSITY OF WESTERN CAPE |
| 5 | 25.35 | -: SEBOGODI - BASETSANA | UNIVERSITY OF FREE STATE |
| 6 | 28.32 | -: MARELETSE - NEO | VAAL UNIVERSITY OF TECHNOLOGY |
| 7 | 28.98 | -: SELESHO - KATLEGO | VAAL UNIVERSITY OF TECHNOLOGY |