| Place | Performance | Athlete | Team |
| 1 | 24.52 | Q: MAHLALELA - BONGIWE | TSHWANE UNIV OF TECHNOLOGY |
| 2 | 24.62 | Q: ANGELIQUE - STRYDOM | TSHWANE UNIV OF TECHNOLOGY |
| 3 | 24.91 | Q: VOSLOO - LEONETTE | UNIVERSITY OF PRETORIA A TEAM |
| 4 | 25.27 | -: KOEKEMOER - DAYLE | USSA INDIVIDUALS (V) |
| 5 | 25.73 | -: KOTZE - DANE | UNIVERSITY OF JOHANNESBURG B |
| 6 | 27.38 | -: VAN STADEN - ANJE | UNIVERSITY OF FREE STATE |
| 7 | 30.35 | -: KUNENE - BOTSHELO TSHIAMISO | RHODES UNIVERSITY |