| Place | Performance | Athlete | Team |
| 1 | 24.32 | Q: J V RENSBURG - SHERE | NORTH WEST UNIVERSITY POTCH A |
| 2 | 25.02 | Q: DE BEER - MIA | UNIVERSITY OF JOHANNESBURG B |
| 3 | 25.32 | Q: COLLINS - YAVE | UNIVERSITY OF PRETORIA B TEAM |
| 4 | 25.76 | -: SANDERS - SHAYLI | UNIVERSITY OF JOHANNESBURG B |
| 5 | 26.91 | -: IMAAN - KHANYISILE | TSHWANE UNIV OF TECHNOLOGY |
| 6 | 27.36 | -: SEMETSI - LINDOKUHLE | TSHWANE UNIV OF TECHNOLOGY |
| 7 | 30.80 | -: MAMPANE - CATHRENE JABULILE | UNIVERSITY OF FORT HARE |