| Place | Performance | Athlete | Team |
| 1 | 24.50 | Q: GIBBON - NICOLA | UNIVERSITY OF FREE STATE |
| 2 | 24.75 | Q: SCHEEPERS - COLENE | UNIVERSITY OF PRETORIA A TEAM |
| 3 | 24.80 | Q: NEL - RHODE | UNIVERSITY OF PRETORIA A TEAM |
| 4 | 25.27 | -: BAYMAN - LAURE | UNIVERSITY OF WESTERN CAPE |
| 5 | 25.36 | -: NDLELA - MELODY THANDA | DURBAN UNIVERSITY OF TECHNOLOGY |
| 6 | 26.16 | -: ACKERMAN - LIZE | UNIVERSITY OF FREE STATE |
| 7 | 26.95 | -: CHAMBISSO - LOLA | VAAL UNIVERSITY OF TECHNOLOGY |