| Place | Performance | Athlete | Team |
| 1 | 23.87 | Q: GEORGE - ANNESTAYSHA | UNIVERSITY OF PRETORIA A TEAM |
| 2 | 24.36 | Q: MARAIS - GABRIELLA | UNIVERSITY OF FREE STATE |
| 3 | 24.82 | Q: PADI - RORISANG | UNIVERSITY OF JOHANNESBURG A |
| 4 | 24.96 | -: FAGBEMI - AMEENAT | UNIVERSITY OF JOHANNESBURG A |
| 5 | 25.15 | -: ROTHMAN - AMIEL | UNIVERSITY OF PRETORIA B TEAM |
| 6 | 25.48 | -: ALECIA - KEYS | UNIVERSITY OF WESTERN CAPE |
| 7 | 25.59 | -: DU PLESSIS - STEFANI | STELLENBOSCH UNIVERSITY |
| 8 | 30.83 | -: NOMBAKUSE - LITHALETHU | WALTER SISULU UNIVERSITY |