| Place | Performance | Athlete | Team |
| 1 | 24.21 | Q: VERMEULEN - CHANE | UNIVERSITY OF JOHANNESBURG A |
| 2 | 24.32 | Q: COMPAAN - MILA | UNIVERSITY OF PRETORIA B TEAM |
| 3 | 24.37 | Q: SMIDT - CHARDONE | NORTH WEST UNIVERSITY POTCH A |
| 4 | 24.94 | q: GERMISHUYEN - EMMARENTIA | UNIVERSITY OF JOHANNESBURG A |
| 5 | 26.60 | -: MACHABA - PORTIA | SOL PLAATJE UNIVERSITY |