| Place | Performance | Athlete | Team |
| 1 | 0:46.07 | Q: JONKER - MARNUS | NORTH WEST UNIVERSITY POTCH A |
| 2 | 0:46.89 | Q: VAN DE WALT - TJ | UNIVERSITY OF JOHANNESBURG A |
| 3 | 0:46.93 | -: MBALULA - LONWABO | NELSON MANDELA UNIVERSITY |
| 4 | 0:47.89 | -: SAMANGA - ANGELO | UNIVERSITY OF WESTERN CAPE |
| 5 | 0:48.48 | -: SAKWE - OLOTHANDO | UNIVERSITY OF JOHANNESBURG B |
| 6 | 0:48.73 | -: MOKHABELA - KAMOHELO | CENTRAL UNIV OF TECHN BLOEMFONTEIN |
| 7 | 0:49.30 | -: MOSIKILI - MOFANA | VAAL UNIVERSITY OF TECHNOLOGY |