| Place | Performance | Athlete | Team |
| 1 | 0:47.89 | Q: VAN RENSBURG - WERNICH | UNIVERSITY OF FREE STATE |
| 2 | 0:48.14 | Q: GORA - LINDUKUHLE | USSA INDIVIDUAL |
| 3 | 0:48.14 | Q: RAMMUPUDU - RORISANG | TSHWANE UNIV OF TECHNOLOGY |
| 4 | 0:48.17 | -: OOSTHUIZEN - RUAN JAN | USSA INDIVIDUALS (V) |
| 5 | 0:52.22 | -: MAREE - FRANCO | UNIVERSITY OF PRETORIA B TEAM |
| 6 | 0:55.18 | -: MASHIGO - LESEDI | SOL PLAATJE UNIVERSITY |