| Place | Performance | Athlete | Team |
| 1 | 0:46.79 | Q: JONKER - MARNUS | NORTH WEST UNIVERSITY POTCH A |
| 2 | 0:47.19 | Q: MASEKO - NHLANHLA | USSA INDIVIDUAL |
| 3 | 0:47.26 | Q: MBALULA - LONWABO | NELSON MANDELA UNIVERSITY |
| 4 | 0:48.87 | -: TITUS - ELRICH | STELLENBOSCH UNIVERSITY |
| 5 | 0:49.78 | -: GLOBBER - GERT HENDRIK | TSHWANE UNIV OF TECHNOLOGY |
| 6 | 0:52.54 | -: MOKGAWA - SELAELO | CENTRAL UNIV OF TECHN BLOEMFONTEIN |
| 7 | 0:53.18 | -: TEBBUTT - CODY | UNIVERSITY OF CAPE TOWN |
| 8 | 0:55.59 | -: MOHLAHLO - KEABETSWE | WALTER SISULU UNIVERSITY |