| Place | Performance | Athlete | Team |
| 1 | 40.56 | ACHURCH - KAYLIN | AFRIKAANS HOR MEISIES |
| 2 | 41.45 | DE BEER - IZEL | MENLOPARK |
| 3 | 41.92 | COLLETT - JASMINE | CURRO HAZELDEAN |
| 4 | 43.03 | DE WET - CHARNE | MENLOPARK |
| 5 | 44.07 | PRETORIUS - LISA | MENLOPARK |
| 6 | 44.16 | FICK - ROXY - LEE | MENLOPARK |
| 7 | 44.33 | ACKERMAN - CARLA | WATERKLOOF |
| 8 | 44.87 | BARRY - KRISTEN | CURRO HAZELDEAN |
| 9 | 45.01 | GILLESPIE - ADDISON | GARSFONTEIN |