| Place | Performance | Athlete | Team |
| 1 | 0:43.53 | DU TOIT - LENKA | HELPMEKAAR |
| 2 | 0:46.84 | LUES - HAYLEY | POTCHEFSTROOM GIMNASIUM |
| 3 | 0:47.36 | DE BRUYN - LOULENE | HELPMEKAAR UITNODIGING |
| 4 | 0:47.54 | PRINS - SHINELL | KLERKSDORP |
| 5 | 0:48.58 | STOLZ - KAYLEEN | HTS MIDDELBURG |
| 6 | 0:48.71 | VAN WYK - CLEO | KEMPTON PARK |
| 7 | 0:48.74 | DU PLESSIS - DANIKA | MENLOPARK UITNODIGING |
| 8 | 0:48.99 | VD WALT - SUMI | RUSTENBURG |
| 9 | 0:49.19 | STEENKAMP - LENIE | FRIKKIE MEYER |
| 10 | 0:49.33 | HEYNS - LINSAY | MENLOPARK UITNODIGING |
| 11 | 0:50.28 | STEYN - MIA | HELPMEKAAR UITNODIGING |
| 12 | 0:51.05 | BADENHORST - JESS | NELSPRUIT |
| 13 | 0:51.62 | MALULEKA - NKATEKO | JEUGLAND |
| 14 | 0:51.73 | MANS - MIEKE | HOR VOLKSKOOL POTCHEFSTROOM |
| 15 | 0:51.75 | BELLINGAN - HAZEL-MARI | SECUNDA |
| 16 | 0:52.13 | ANDERSON-KRIEL - CATHERINE | RANDBURG |
| 17 | 0:52.27 | BOERS - LILY | NORTHCLIFF HIGH |
| 18 | 0:52.40 | STRASSBURG - LEYNA | HOR VOLKSKOOL HEIDELBERG |
| 19 | 0:52.64 | LANGEVELDT - MARLI | ELDORAIGNE |
| 20 | 0:52.67 | JONES - NIKITA | MONTANA UITNODIGING |
| 21 | 0:53.26 | JACOBS - IVANKE | HARTBEESPOORT |
| 22 | 0:53.40 | CRONJE - MELINIQUE | RIEBEECKRAND |
| 23 | 0:54.41 | LABUSCHAGNE - JEAN-NIKA | HARTBEESPOORT |
| 24 | 0:54.79 | VD BANK - LOGAN | DRIEHOEK |
| 25 | 0:55.00 | THIERZEN - SUZAAN | OVERVAAL |
| 26 | 0:55.41 | NONYANE - HEILEE | JEUGLAND |
| 27 | 0:56.81 | DOLLAR - JORDAN | FLORIDA |
| 28 | 1:01.87 | BOUSFIELD - MACKAYLA | DIE ANKER |
| 29 | 1:04.42 | MABITSELA - CHRISTINA | JAN VILJOEN |