| Place | Performance | Athlete | Team |
| 1 | 27.62 | q: DU PLESSIS - DANIKA | LAERSKOOL MENLOPARK |
| 2 | 28.48 | q: LABUSCHAGNE - JEAN-NIKA | JS ATHLETICS |
| 3 | 29.05 | -: BURGER - LIVIA | WILLEBEER |
| 4 | 29.66 | -: ERASMUS - MILAN | LAERSKOOL BAKENKOP |
| 5 | 29.68 | -: MOSELAKGOMO - REGOIKANTSE | SESHEGONG SEC SCHOOL DEVELOPMENT |
| 6 | 30.51 | -: MBALEHLE - ASHLEIGH | OUR VOICE ATHLETICS CLUB |