| Place | Performance | Athlete | Team |
| 1 | 26.99 | q: GOVENDER - KERIANA | CURRO HAZELDEAN |
| 2 | 27.78 | q: KRUGER - MIKAELA | JS ATHLETICS |
| 3 | 27.83 | q: THOTHELA - NEO | JS ATHLETICS |
| 4 | 28.92 | -: VLOK - LYNN | LAERSKOOL HENNOPSPARK |
| 5 | 29.12 | -: HLOBO - LIKANO | JS ATHLETICS |
| 6 | 30.47 | -: BOOYS - TAMARA | COURAGEOUS ATHLETICS DEVELOPMENT |
| 7 | 30.63 | -: NGUGI - NATALIE | JS ATHLETICS |