| Place | Performance | Athlete | Team |
| 1 | 25.38 | VAN DER MERWE - ZANTE | AFRIKAANS HOR MEISIES BLOU |
| 2 | 25.44 | MAKASI - LOLWETHU | INDIVIDUAL ENTRIES |
| 3 | 26.22 | BOTES - ZENIQUE | AFRIKAANS HOR MEISIES BLOU |
| 4 | 26.55 | MADLAKUVANA - SISIPHO | TUKS SPORT |
| 5 | 26.57 | NIEMANDT - KARA | CURRO HAZELDEAN |
| 6 | 26.67 | PULE - MPHO | CURRO HAZELDEAN |
| 7 | 26.75 | MGQIBELA - BUHLE | TUKS SPORT |
| 8 | 27.14 | MAKADI - DAVIDA | PRETORIA HIGH SCHOOL FOR GIRLS |
| 9 | 27.19 | DE BEER - ARETHA | HORSKOOL OOS-MOOT |
| 10 | 27.41 | VAN JAARSVELD - SHANIQUE | HORSKOOL GARSFONTEIN |
| 11 | 27.56 | PRETORIUS - CALEIGH | HORSKOOL ZWARTKOP |
| 12 | 27.77 | MINNAAR - HELINE | HORSKOOL MENLOPARK WIT |
| 13 | 27.92 | BREYTENBACH - ANNELET | HORSKOOL OOS-MOOT |
| 14 | 27.94 | VAN DER WESTHUIZEN - ELZAAN | MIDSTREAM COLLEGE |
| 15 | 28.18 | CRAFFORD - DIHANE | HORSKOOL ZWARTKOP |
| 16 | 28.46 | MKANSI - LEBOGANG | THE GLEN HIGH SCHOOL |
| 17 | 28.67 | VERMAAK - CAITLYN | EDUPLEX |
| 18 | 28.77 | STRUWIG - CHRISTELLE | MIDSTREAM COLLEGE |
| 19 | 28.80 | KEWANA - AZI | AMBERFIELD COLLEGE |
| 20 | 29.18 | MARITZ - ABINICA | HORSKOOL WONDERBOOM |
| 21 | 30.06 | BARNES - CHARICE | PRETORIA HIGH SCHOOL FOR GIRLS |
| 22 | 30.22 | KGOWEDI - MOTHEO | SUTHERLAND HIGH SCHOOL |
| 23 | 30.38 | MABIDI - FAITH | CURRO THATCHFIELD |
| 24 | 31.52 | PHOSOKO - TIISETSO | AMBERFIELD COLLEGE |
| 25 | 31.53 | MTHOMBENI - NIKIWE | REDDFORD HOUSE THE HILLS |