| Place | Performance | Athlete | Team |
| 1 | 0:42.86 | NIEMAN - MEGAN | HORSKOOL MENLOPARK BLOU |
| 2 | 0:44.45 | GUNTER - ISABELLA | MONTANA HORSKOOL |
| 3 | 0:45.48 | BOUWER - KARLA | HORSKOOL GARSFONTEIN |
| 4 | 0:46.37 | LOGGENBERG - CHRISTI | AFRIKAANS HOR MEISIES BLOU |
| 5 | 0:46.79 | BREWIS - BIANCA | HORSKOOL WATERKLOOF |
| 6 | 0:47.14 | HUGO - SIMONNE | AFRIKAANS HOR MEISIES BLOU |
| 7 | 0:48.34 | VANDER ENDE - RUVE | HORSKOOL MENLOPARK BLOU |
| 8 | 0:48.85 | VAN STADEN - ZOE | HORSKOOL MENLOPARK BLOU |
| 9 | 0:49.23 | SEDUPANE - BOKAMOSO | CURRO HAZELDEAN |
| 10 | 0:53.95 | PRETORIUS - ANRI | MIDSTREAM COLLEGE |
| 11 | 0:54.10 | ZAAIMAN - MEGAN | HORSKOOL MENLOPARK WIT |
| 12 | 0:54.31 | SCHULTZ - CARA | HORSKOOL MENLOPARK WIT |
| 13 | 0:54.52 | PRETORIUS - AN | HORSKOOL WATERKLOOF |
| 14 | 0:56.90 | FATOKI - OYIN | CURRO HAZELDEAN |