| Place | Performance | Athlete | Team |
| 1 | 24.48 | Q: BASSON - KYLIE | BOLAND ATHLETICS |
| 2 | 24.58 | Q: BURGER - RUM | ATHLETICS FREE STATE |
| 3 | 24.90 | Q: CILLIERS - ZO | ATHLETICS MPUMALANGA |
| 4 | 25.01 | -: VAN DYK - JENNA | ATHLETICS CENTRAL NORTHWEST |
| 5 | 25.13 | -: BOTES - ZENIQUE | ATHLETICS GAUTENG NORTH |
| 6 | 25.23 | -: VAN STADEN - MICHE | WESTERN PROVINCE ATHLETICS |
| 7 | 25.68 | -: FOURIE - ASTRID | ATHLETICS VAAL TRIANGLE |
| 8 | 25.77 | -: PRETORIUS - LISA | ATHLETICS GAUTENG NORTH |