| Place | Performance | Athlete | Team |
| 1 | 24.37 | Q: VAN OUDTSHOORN - KAYLIE | CENTRAL GAUTENG ATHLETICS |
| 2 | 24.37 | Q: VERMAAK - HANNAH HOPE | CENTRAL GAUTENG ATHLETICS |
| 3 | 24.66 | Q: VAN DER MERWE - ZANTE | ATHLETICS GAUTENG NORTH |
| 4 | 25.09 | -: DU PLOOY - LUANNE | CENTRAL GAUTENG ATHLETICS |
| 5 | 25.23 | -: MAKASI - LOLWETHU | CENTRAL GAUTENG ATHLETICS |
| 6 | 25.49 | -: FOUCHE - KEIRA | CENTRAL GAUTENG ATHLETICS |
| 7 | 25.53 | -: VAN DER WESTHUYZEN - ELLENE | LIMPOPO ATHLETICS |
| 8 | 25.80 | -: MAREE - KYLA (KYLA - MAE) | ATHLETICS CENTRAL NORTHWEST |