| Place | Performance | Athlete | Team |
| 1 | 24.62 | Q: GUNTER - ISABELLA | ATHLETICS GAUTENG NORTH |
| 2 | 24.72 | Q: KONDE - FAITH | BOLAND ATHLETICS |
| 3 | 24.89 | Q: PRETORIUS - ANE | ATHLETICS GAUTENG NORTH |
| 4 | 25.02 | -: CORNELIUS - ASHLEY | BOLAND ATHLETICS |
| 5 | 25.34 | -: MAAKE - MOKGADI | ATHLETICS GAUTENG NORTH |
| 6 | 25.44 | -: BOTMA - CHENADE | ATHLETICS MPUMALANGA |
| 7 | 25.77 | -: LOGGENBERG - NINA | ATHLETICS GAUTENG NORTH |