| Place | Performance | Athlete | Team |
| 1 | 25.13 | Q: NDUBUEZE - OLUCHI ABELWE | CENTRAL GAUTENG ATHLETICS |
| 2 | 25.17 | Q: KOOPMAN - TAYGEN | WESTERN PROVINCE ATHLETICS |
| 3 | 25.46 | Q: KAYSER - CHARNE | BOLAND ATHLETICS |
| 4 | 25.48 | -: VAN DER MERWE - DENE | ATHLETICS GAUTENG NORTH |
| 5 | 25.57 | -: VAN DER WESTHUYZEN - EMMA-LEE | LIMPOPO ATHLETICS |
| 6 | 25.58 | -: REED - SARAH | KWAZULU-NATAL ATHLETICS |
| 7 | 26.20 | -: OKOYE - PARIS | CENTRAL GAUTENG ATHLETICS |