| Place | Performance | Athlete | Team |
| 1 | 24.30 | Q: LA GRANGE - KAYLA | CENTRAL GAUTENG ATHLETICS |
| 2 | 24.63 | Q: MARIAS - GABRIELLA | ATHLETICS FREE STATE |
| 3 | 24.89 | Q: COMPAAN - MILA | ATHLETICS GAUTENG NORTH |
| 4 | 25.14 | q: OOSTHUIZEN - SYDNEY | ATHLETICS CENTRAL NORTHWEST |
| 5 | 25.55 | -: STOLZ - JENNA | CENTRAL GAUTENG ATHLETICS |
| 6 | 25.77 | -: KEYS - ALECIA | WESTERN PROVINCE ATHLETICS |