| Place | Performance | Athlete | Team |
| 1 | 24.40 | Q: GEORGE - ANNESTAYSHA | ATHLETICS GAUTENG NORTH |
| 2 | 24.47 | Q: LE ROUX - KAYLEE | CENTRAL GAUTENG ATHLETICS |
| 3 | 25.22 | Q: SCHOONBEE - BIANCA | EASTERN PROVINCE ATHLETICS |
| 4 | 25.28 | q: ROTHMAN - AMIEL | ATHLETICS CENTRAL NORTHWEST |
| 5 | 25.29 | -: NDLELA - MELODY | KWAZULU-NATAL ATHLETICS |
| 6 | 25.52 | -: MBOMBO - ALIYAH | WESTERN PROVINCE ATHLETICS |
| 7 | 26.38 | -: BANUTELO - SOPHIE | ATHLETICS GAUTENG NORTH |