| Place | Performance | Athlete | Team |
| 1 | 23.77 | Q: BOTJE - KAILI | CENTRAL GAUTENG ATHLETICS |
| 2 | 24.41 | Q: COETZEE - TIMEKE JADE | ATHLETICS GAUTENG NORTH |
| 3 | 25.38 | -: VILANKULU - ALUWANI | ATHLETICS FREE STATE |
| 4 | 25.47 | -: VAN HEERDEN - JESSICA | ATHLETICS CENTRAL NORTHWEST |
| 5 | 25.69 | -: KARRIEM - NABOEWEYAH | WESTERN PROVINCE ATHLETICS |
| 6 | 26.20 | -: THALE - KAMOGELO | ATHLETICS NORTHWEST NORTH |