| Place | Performance | Athlete | Team |
| 1 | 23.91 | Q: WANSBURY - CHARN | ATHLETICS FREE STATE |
| 2 | 24.06 | Q: VERMEULEN - CHANE | CENTRAL GAUTENG ATHLETICS |
| 3 | 24.95 | q: PETERSEN - ANGIE (IND) | BOLAND ATHLETICS |
| 4 | 25.67 | -: KIRSTEN - BRENDA | ATHLETICS MPUMALANGA |
| 5 | 26.43 | -: SEMONO - THATO | ATHLETICS GAUTENG NORTH |
| 6 | 27.45 | -: CLOETE - RIONA | NORTHWESTERN CAPE ATHLETICS |