| Place | Performance | Athlete | Team |
| 1 | 23.60 | Q: JINGQI - VIWE | ATHLETICS CENTRAL NORTHWEST |
| 2 | 24.21 | Q: MOLEPO - PRECIOUS | CENTRAL GAUTENG ATHLETICS |
| 3 | 24.90 | q: SCHEEPERS - COLENE | ATHLETICS GAUTENG NORTH |
| 4 | 25.42 | -: PRETORIUS - EMKE | ATHLETICS MPUMALANGA |
| 5 | 26.10 | -: THALE - AMOGELANG | ATHLETICS NORTHWEST NORTH |
| 6 | 27.44 | -: LUBOWA - PRECIOUS SANELISIWE | ATHLETICS TRANSKEI |