| Place | Performance | Athlete | Team |
| 1 | 21.16 | Q: OLIVIER - ANDRE | ATHLETICS GAUTENG NORTH |
| 2 | 21.45 | Q: ELAND - ROBYN-LEE | ATHLETICS GRIQUALAND WEST |
| 3 | 21.85 | Q: SCHEEPERS - DYLAN | CENTRAL GAUTENG ATHLETICS |
| 4 | 22.05 | q: TSHELANE - TSHELANE | ATHLETICS NORTHWEST NORTH |
| 5 | 22.22 | q: CHILOANE - LEHLOGONOLO | ATHLETICS MPUMALANGA |
| 6 | 22.87 | -: TSHEBISO - THABANG | ATHLETICS CENTRAL NORTHWEST |
| 7 | 23.09 | -: NOKERI - PHILLIP | LIMPOPO ATHLETICS |