| Place | Performance | Athlete | Team |
| 1 | 24.10 | Q: VAN OUDTSHOORN - KAYLIE | CENTRAL GAUTENG ATHLETICS |
| 2 | 24.34 | Q: BASSON - KYLIE | BOLAND ATHLETICS |
| 3 | 25.26 | Q: DU PLOOY - LUANNE | CENTRAL GAUTENG ATHLETICS |
| 4 | 25.64 | q: FOURIE - ASTRID | ATHLETICS VAAL TRIANGLE |
| 5 | 26.00 | -: VAN WYK - ANIKA | ATHLETICS FREE STATE |
| 6 | 27.05 | -: HINKS - TYLA | EASTERN PROVINCE ATHLETICS |
| 7 | 32.09 | -: MKENTANE - ONWABA IKO | ATHLETICS TRANSKEI |